| PRS 0209-912-Neira
Neira R, Arroyave J, Ramirez H, Ortiz CL, Solarte E, Sequeda F, Gutierrez MI.
Fat liquefaction: effect of low-level laser energy on adipose tissue.
Plast Reconstr Surg. 2002 Sep 1;110(3):912-22; discussion 923-5.
Department of Plastic Surgery, Centro Medico Imbanaco, Avenida 4-Oeste #5-274,
Apto 301 B, Edificia Bosque Valladares, Cali, Colombia. neira-rod@emcali.net.co
Low-level laser energy has been increasingly used in the treatment of a broad
range of conditions and has improved wound healing, reduced edema, and relieved
pain of various etiologies. This study examined whether 635-nm low-level lasers
had an effect on adipose tissue in vivo and the procedural implementation of
lipoplasty/liposuction techniques. The experiment investigated the effect of
635-nm, 10-mW diode laser radiation with exclusive energy dispersing optics.
Total energy values of 1.2 J/cm(2), 2.4 J/cm(2), and 3.6 J/cm(2) were applied on
human adipose tissue taken from lipectomy samples of 12 healthy women. The
tissue samples were irradiated for 0, 2, 4, and 6 minutes with and without
tumescent solution and were studied using the protocols of transmission electron
microscopy and scanning electron microscopy. Nonirradiated tissue samples were
taken for reference. More than 180 images were recorded and professionally
evaluated. All microscopic results showed that without laser exposure the normal
adipose tissue appeared as a grape-shaped node. After 4 minutes of laser
exposure, 80 percent of the fat was released from the adipose cells; at 6
minutes of laser exposure, 99 percent of the fat was released from the
adipocyte. The released fat was collected in the interstitial space.
Transmission electron microscopic images of the adipose tissue taken at x60,000
showed a transitory pore and complete deflation of the adipocytes. The low-level
laser energy affected the adipose cell by causing a transitory pore in the cell
membrane to open, which permitted the fat content to go from inside to outside
the cell. The cells in the interstitial space and the capillaries remained
intact. Low-level laser-assisted lipoplasty has a significant impact on the
procedural implementation of lipoplasty techniques.
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