| PRS 0405-1796 Brown
Brown SA, Rohrich RJ, Kenkel J, Young VL, Hoopman J, Coimbra M.
Effect of low-level laser therapy on abdominal adipocytes before lipoplasty
procedures. Plast Reconstr Surg. 2004 May;113(6):1796-804; discussion 1805-6.
Nancy Lee and Perry Bass Advanced Wound Healing and Tissue Regeneration
Laboratory, Department of Plastic Surgery, University of Texas Southwestern
Medical Center, 5323 Harry Hines Boulevard, Dallas, TX 75390-9132, USA.
spencer.brown@utsouthwestern.edu
Low-level laser therapy is a new subspecialty for the medical application of
lasers that provides therapeutic rather than surgical outcomes for many medical
indications. Recently, low-level laser therapy was reported to "liquefy" or
release stored fat in adipocytes by the opening of specialized yet not
identified cell membrane-associated pores after a brief treatment. Currently,
low-level laser therapy is a U.S. Food and HYPERLINK "http://click.linksynergy.com/fs-bin/click?id=C3sqBBWXXlg&offerid=52722.10000005&type=3&subid=0" drug Administration-approved
technology for improving pain alleviation. To explore these data further, a
series of in vitro studies on human preadipocytes and institutional animal care
and use committee-approved protocols in a porcine Yucatan model and an
institutional review board-approved clinical study were performed. Using a
635-nm low-level laser of 1.0 J/cm supplied to the authors by the vendor, these
studies were designed to determine whether alteration in adipocyte structure or
function was modulated after low-level laser therapy. Cultured human
preadipocytes after 60 minutes of laser therapy did not change appearance
compared with nonirradiated control cells. In the porcine model, low-level laser
therapy (30 minutes) was compared with traditional lipoplasty (suction-assisted
lipoplasty) and ultrasound-assisted lipoplasty. From histologic and scanning
electron microscopic evaluations of the lipoaspirates, no differences were
observed between low-level laser therapy-derived and suction-assisted
lipoplasty-derived specimens. Using exposure times of 0, 15, 30, and 60 minutes
in the presence or absence of superwet wetting solution and in the absence of
lipoplasty, total energy values of 0.9 mW were delivered to tissue samples at
three increasing depths from each experimental site. No histologic tissue
changes or specifically in adipocyte structure were observed at any depth with
the longest low-level laser therapy (60 minutes with superwet fluid). Three
subjects undergoing large-volume lipoplasty were exposed to superwet wetting
fluid infiltration 14 minutes before and 12 minutes after, according to vendor
instructions. Tissue samples from infiltrated areas were collected before
suction-assisted lipoplasty and lipoaspirates from suction-assisted lipoplasty.
No consistent observations of adipocyte disruptions were observed in the
histologic or scanning electron microscopy photographs. These data do not
support the belief that low-level laser therapy treatment before lipoplasty
procedures disrupts tissue adipocyte structure.
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